Restriction insensitivity in bacteriophage T5. II. Lack of EcoRI modification in T5+ and T5ris mutants
نویسندگان
چکیده
منابع مشابه
Genetic suppressors of bacteriophage t5 amber mutants.
Nonsense mutations in structural genes appear to abort polypeptide chain synthesis at the amino acid site governed by the mutant codon. The two presently recognized types of nonsense mutant, amber and ochre, have been assigned the codons UAG and UAA, respectively. Certain bacteria harbor one or more genetic suppressors that counteract the effect of appropriate nonsense mutations in their own st...
متن کاملMapping of Temperature-sensitive Mutants in Bacteriophage T5.
5, one of the classical T phages of Escherichia coli, has been the subject of many studies, but its genetic analysis has been sporadic. Beginning with heatstable ( s t ) mutants (ADAMS and LARK 1950; ADAMS 1953), there have been reports of mutants with altered host range (LANNI and LANNI 1956), serological behavior (LANNI and LANNI 1957), lysis-inhibiting properties (U. T. LANNI 1958), and buoy...
متن کاملPolarized injection of the bacteriophage T5 chromosome.
Examination of the first-step-transfer DNA of T5ris mutants which carry new EcoRI sites showed that the left end of the chromosome is injected first.
متن کاملRole of the T5 gene D15 nuclease in the generation of nicked bacteriophage T5 DNA.
The processing of newly replicated concatameric T5 DNA into both single stranded DNA changed of unit length and single-stranded fragments of sizes comparable to those found in mature T5 virion DNA occurs in the absence of late T5 protein synthesis. The formation of unit-length, single-stranded DNA chains does not require the early T5 gene D15 nuclease: however, the subsequent formation of the s...
متن کاملInterruption-deficient mutants of bacteriophage T5: analysis of single-site mutants.
The properties of viable mutants of bacteriophage T5 that lack, singly, each of the four major sites at which single-chain interruptions normally occur in T5 DNA are described. The mutations responsible for loss of each interruption were mapped by analysis with HhaI, a restriction endonuclease with a cleavage site (pGCGC) that occurs at the 5' termini of the major interruptions (B. P. Nichols a...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1979
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.29.1.17-20.1979